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Catalog Number | orb706814 |
---|---|
Category | Proteins |
Description | Recombinant Human BTN2A1 Protein is produced by mammalian expression system and the target gene encoding Gln29-Ala248 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | BTN2A1 |
Entrez | 11120 |
UniProt ID | Q7KYR7 |
Protein Sequence | QFIVVGPTDP ILATVGENTT LRCHLSPEKN AEDMEVRWFR SQFSPAVFVY KGGRERTEEQ MEEYRGRTTF VSKDISRGSV ALVIHNITAQ ENGTYRCYFQ EGRSYDEAIL HLVVAGLGSK PLISMRGHED GGIRLECISR GWYPKPLTVW RDPYGGVAPA LKEVSMPDAD GLFMVTTAVI IRDKSVRNMS CSINNTLLGQ KKESVIFIPE SFMPSVSPCA HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | BT2.1 protein, BTF1 protein, Butyrophilin subfamil Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
26.6 kDa | |
Yeast |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 25.4 kDa after removal of the signal peptide. The apparent molecular mass of BTN2A1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
26.4 kDa | |
Human BTN2A1/Butyrophilin, His Tag (orb867306) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Ala 248 (Accession # Q7KYR7-2). |
98.00% | |
39-44 KDa, reducing conditions |
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