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Catalog Number | orb257199 |
---|---|
Category | Proteins |
Description | Human beta 2-Microglobulin Protein, His Tag |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 98% |
Conjugation | Unconjugated |
MW | 12.6 kDa |
Target | beta 2-Microglobulin |
UniProt ID | P61769 |
Protein Sequence | AAH32589.1 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ile 21 - Met 119 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH32589.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 12.6 kDa. The protein migrates as 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human beta 2-Microglobulin protein
98.00% | |
35.8 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
98.10% | |
13.2 kDa (predicted); 13.2 kDa (reducing conditions) |
≥90% as determined by SDS-PAGE | |
This protein contains the human B2M(Ile21-Met119) was fused with the N-terminal His Tag and expressed in E. coli. |
> 90%, determined by SDS-PAGE | |
This protein contains the human B2M (NP_0040391) (Met 1-Met 119) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 97%, determined by SDS-PAGE | |
This protein contains the human B2M (NP_0040391) (Met 1-Met 119) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |