Human BDH1 protein

SKU: orb391290

Description

Recombinant human BDH1 protein is produced by our E. coli expression system and the target gene encoding Met1-Arg343 is expressed with a 6His tag at the N-terminus.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: BDH1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA EPVGSKAVLV TGCDSGFGFA LAKHLHSKGF LVFAGCLMKD KGHDGVKELD SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 50mM Tris, 1mM DTT, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BDH; D-beta-hydroxybutyrate dehydrogenase mitochondrial; BDH; 3-hydroxybutyrate dehydrogenase

Quick Database Links

Gene Symbol

BDH1

UniProt

Q02338

NCBI Gene ID

622