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Catalog Number | orb391290 |
---|---|
Category | Proteins |
Description | Recombinant human BDH1 protein is produced by our E. coli expression system and the target gene encoding Met1-Arg343 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM Tris, 1mM DTT, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | BDH1 |
Entrez | 622 |
UniProt ID | Q02338 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA EPVGSKAVLV TGCDSGFGFA LAKHLHSKGF LVFAGCLMKD KGHDGVKELD SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM Tris, 1mM DTT, pH 7.5. |
Alternative names | BDH; D-beta-hydroxybutyrate dehydrogenase mitochon Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
37.1 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human BDH1(Met1-Arg343) was fused with the N-terminal His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
37.6 kDa | |
E.Coli |
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