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Catalog Number | orb391277 |
---|---|
Category | Proteins |
Description | Recombinant human B4GALT3 protein is produced by our mammalian expression system and the target gene encoding Arg32-His393 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 2mM MgCl2, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | B4GALT3 |
Entrez | 8703 |
UniProt ID | O60512 |
Protein Sequence | RSLSALFGRD QGPTFDYSHP RDVYSNLSHL PGAPGGPPAP QGLPYCPERS PLLVGPVSVS FSPVPSLAEI VERNPRVEPG GRYRPAGCEP RSRTAIIVPH RAREHHLRLL LYHLHPFLQR QQLAYGIYVI HQAGNGTFNR AKLLNVGVRE ALRDEEWDCL FLHDVDLLPE NDHNLYVCDP RGPRHVAVAM NKFGYSLPYP QYFGGVSALT PDQYLKMNGF PNEYWGWGGE DDDIATRVRL AGMKISRPPT SVGHYKMVKH RGDKGNEENP HRFDLLVRTQ NSWTQDGMNS LTYQLLAREL GPLYTNITAD IGTDPRGPRA PSGPRYPPGS SQAFRQEMLQ RRPPARPGPL STANHTALRG SHVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 2mM MgCl2, 10% Glycerol, pH 7.5. |
Alternative names | Beta-1,4-galactosyltransferase 3; Beta-1,4-GalTase Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
67.2 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human B4GALT3(Arg32-His393) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
98.00% | |
50 KDa, reducing conditions |
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