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Catalog Number | orb391225 |
---|---|
Category | Proteins |
Description | Recombinant human AMY2B protein is produced by our mammalian expression system and the target gene encoding Gln16-Leu511 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | AMY2B |
Entrez | 280 |
UniProt ID | P19961 |
Protein Sequence | QYSPNTQQGR TSIVHLFEWR WVDIALECER YLAPKGFGGV QVSPPNENVA IHNPFRPWWE RYQPVSYKLC TRSGNEDEFR NMVTRCNNVG VRIYVDAVIN HMSGNAVSAG TSSTCGSYFN PGSRDFPAVP YSGWDFNDGK CKTGSGDIEN YNDATQVRDC RLVGLLDLAL EKDYVRSKIA EYMNHLIDIG VAGFRLDASK HMWPGDIKAI LDKLHNLNSN WFPAGSKPFI YQEVIDLGGE PIKSSDYFGN GRVTEFKYGA KLGTVIRKWN GEKMSYLKNW GEGWGFMPSD RALVFVDNHD NQRGHGAGGA SILTFWDARL YKMAVGFMLA HPYGFTRVMS SYRWPRQFQN GNDVNDWVGP PNNNGVIKEV TINPDTTCGN DWVCEHRWRQ IRNMVNFRNV VDGQPFTNWY DNGSNQVAFG RGNRGFIVFN NDDWTFSLTL QTGLPAGTYC DVISGDKING NCTGIKIYVS DDGKAHFSIS NSAEDPFIAI HAESKLVDHH HHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Alpha-amylase 2B; 14-alpha-D-glucan glucanohydrola Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human AMY2B(Gln16-Leu511) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
98.00% | |
Approxiamtely 82.4 kDa |
98.00% | |
Approxiamtely 57.3 kDa |
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