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Catalog Number | orb257286 |
---|---|
Category | Proteins |
Description | Human ALCAM, His Tag is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # Q13740-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 57.8 kDa |
Target | ALCAM |
UniProt ID | Q13740 |
Protein Sequence | NP_001618.2 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Trp 28 - Ala 526 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001618.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.8 kDa. The protein migrates as 70-100 kDa under reducing (R) condition, and 66-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human ALCAM protein
98.00% | |
57 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
98.00% | |
58.9 kDa (predicted). Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result. |
SDS-PAGE: 96.5%; SEC-HPLC: 99.2% | |
57.4 kDa (predicted); 78.3 kDa (reducing condition, due to glycosylation) |
> 97% by SDS-PAGE. | |
Recombinant Human ALCAM/CD166 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Ala526) of human ALCAM/CD166 (Accession #NP_001618.2) fused with an Fc, 6��His Tag at the C-terminus. |
> 98%, determined by SDS-PAGE | |
This protein contains (Met 1-Ala 526) of human ALCAM (NP_0016182) was expressed with the C-terminal polyhistidine tag and expressed from HEK293 Cells. |