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Human ADAMTS13 Protein, His Tag
Description
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | ADAMTS13 |
| Reactivity | Monkey |
| Tag | C-6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 150.8 kDa after removal of the signal peptide. The apparent molecular mass of ADAMTS13-His is approximately 130-250 kDa due to glycosylation. |
| Expression Region | ADAMTS13(Gln34-Thr1427)+6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human ADAMTS13 Protein, C-His [orb2832867]
>90% as determined by SDS-PAGE.
76 kDa
20 μg, 50 μg, 100 μgRecombinant Human ADAMTS13 Protein, N-His [orb2835173]
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20.87 kDa
20 μg, 50 μg, 100 μgRecombinant human ADAMTS13 protein,N-His [orb2837162]
>90% as determined by SDS-PAGE
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ELISA plate pre-coated by 2 μg/mL (100 μL/well) Cynomolgus GPRC5D Protein, hFc Tag (orb1173898) can bind Anti-GPRC5D antibody, Rabbit mAb orb1174077 in a linear range of 0.57–76.33 ng/ml.
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UniProt Details
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Protocol Information
Human ADAMTS13 Protein, His Tag (orb2321448)
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