Human Activin A Receptor Type I (ACVR1) ELISA Kit

SKU: orb778325

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type I(ACVR1).

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACVR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACVR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACVR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACVR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ACVR1A ELISA Kit, ALK2 ELISA Kit, ACVRLK2 ELISA Kit, TSRI ELISA Kit, FOP ELISA Kit, SKR1 ELISA Kit, ACTRI ELISA Kit, Activin receptor-like kinase 2 ELISA Kit, Serine/threonine-protein kinase receptor R1 ELISA Kit, TGF-B superfamily receptor type I ELISA Kit

Quick Database Links

UniProt

Q04771