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Human Actin Gamma 2, Smooth Muscle (ACTg2) ELISA Kit

Catalog Number: orb778660

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778660
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Gamma 2, Smooth Muscle(ACTg2).
ReactivityHuman
Concentration10 ng/mL
UniProt IDP63267
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTg2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTg2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTg2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTg2 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.054 ng/mL
Sample TypesTissue homogenates and other biological fluids.
Alternative namesACT ELISA Kit, ACT-G2 ELISA Kit, ACTA3 ELISA Kit,
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NoteFor research use only
Human Actin Gamma 2, Smooth Muscle (ACTg2) ELISA Kit