Human A Disintegrin and Metalloprotease 8 (ADAM8) ELISA Kit

SKU: orb775559

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 8(ADAM8).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADAM8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADAM8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADAM8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADAM8 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 pg/mL
• Sensitivity: 2.46 pg/mL
• Concentration: 400 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD156-A ELISA Kit, MS2 ELISA Kit, CD156a ELISA Kit, Disintegrin and metalloproteinase domain-containing protein 8 ELISA Kit

Quick Database Links

UniProt

P78325