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Catalog Number | orb757424 |
---|---|
Category | Proteins |
Description | Recombinant Human 5T4 Protein with C-terminal 6×His tag |
Reactivity | Human |
Tag | C-6×His Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 36.1 kDa after removal of the signal peptide.The apparent molecular mass of 5T4-His is approximately 55-70 kDa due to glycosylation. |
Target | 5T4 |
UniProt ID | Q13641 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | 5T4;5T4AG;M6P1;WAIF1 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human 4-1BB Protein, His Tag on SDS-PAGE under reducing condition.
Flow cytometry analysis with 1 μg/mL Human 4-1BB Protein, His tag (orb689478) on Expi293 cells transfected with human 4-1BBL (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
98.00% | |
36.15 kDa (predicted); 62.88 kDa (reducing conditions) |
98.00% | |
38.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
60-90 KDa (reducing condition) |
SEC-HPLC: 98.6% | |
33.1 kDa (predicted) |
> 95% by SDS-PAGE. | |
Recombinant Human TPBG Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ser32-Ser355) of human TPBG (Accession #Q13641) fused with a 6��His Tag at the C-terminus. |