Human 14-3-3 Protein epsilon (YWHAE) ELISA Kit

SKU: orb782813

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAE(14-3-3 protein epsilon).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human YWHAE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human YWHAE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human YWHAE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human YWHAE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 26.5 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

14-3-3 E ELISA Kit, 14-3-3 epsilon ELISA Kit, FLJ45465 ELISA Kit, MDCR ELISA Kit, YWHAE ELISA Kit, protein kinase C inhibitor protein 1 ELISA Kit, MDS ELISA Kit, KCIP 1 ELISA Kit

Quick Database Links

UniProt

P62258