HTH-01-015

SKU: orb1305577

Description

HTH-01-015

Research Area

Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 1613724-42-7
• MW: 468.55
• Purity: 98.10%
• Formula: C₂₆H₂₈N₈O
• SMILES: CN1c2cc3ccccc3cc2C(=O)N(C)c2c(C)nc(Nc3cnn(c3)C3CCNCC3)nc12
• Target: AMPK
• Solubility: DMSO:52 mg/mL (110.98 mM);Ethanol:28 mg/mL (59.76 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.13 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  NUAK1:100 nM
• In Vitro:
  In HEK-293 cells express NUAK1 as well as NUAK2, HTH-01-015 suppresses NUAK1-mediated MYPT1 phosphorylation. HTH-01-015 suppresses cell migration In NUAK1+/+ MEFs, and inhibit U2OS cell invasion. Moreover, HTH-01-015 inhibits cell proliferation In both cell lines. HTH-01-015 inhibitors markedly restricted cells from entering into mitosis in U2OS cells.
• Cell Research:
  Cell proliferation assays are carried out colorimetrically in 96-well plates usin the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit followin the manufacturer's protocol. Initially, 2000 cells per well are seeded for U2OS cells and 3000 cells per well are seeded for MEFs the proliferation assays are carried out over 5 days in the presence or absence of 10 μM HTH-01-015.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

AMP-activated protein kinase, AMPK, inhibit, NUAK1, NUAK2, HTH 01 015, HTH01015, HTH-01-015, Inhibitor

Compound Identifiers

PubChem CID

78357766