hsa-miR-486-5p miRNA Agomir

SKU: orb1921324

Description

Synthetic miRNA Agomir is used to regulate the expression of target hsa-miR-486-5p mRNA.

Images & Validation

• Application Notes:
  Components: This synthetic miRNA is based on the mature miRNA sequence. The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo. Directions for Use: Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 5 nmol miRNA agomir to 20 µM using 250 µl DEPC H2O (or RNase-free water). Pipette the solution up and down 3-5 times (or vortex briefly). Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA agomir into small volumes and store at ≤ -20°C. miRNA agomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times

Key Properties

• Target: hsa-miR-486-5p
• Source: Synthetic
• Reactivity: Human

Storage & Handling

• Storage: Shipped at 4 °C. Store at -20 °C for one year. Avoid freeze-thaw cycles after reconstitution.
• Form/Appearance: Liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

hsa-miR-486-5p