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Catalog Number | orb347170 |
---|---|
Category | Antibodies |
Description | Horse IgG (H&L) antibody (Texas Red) |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Texas Red |
Reactivity | Equine |
Form/Appearance | Lyophilized |
Concentration | 2.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum. |
Immunogen | Horse IgG whole molecule |
Tested applications | FC, FLISA, IF, WB |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
Application notes | Anti-Horse IgG Texas Red Antibody has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | Rabbit Anti-Horse IgG Texas Red Conjugated Antibod Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot of Texas Red™ Rabbit Anti-Horse IgG secondary antibody. Lane 1: Horse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Horse IgG. Other band(s): None.
ELISA, FC, IF, IH | |
Equine | |
Rabbit | |
Polyclonal | |
Texas Red |
FC, FLISA, IF | |
Equine | |
Goat | |
Polyclonal | |
Texas Red |
Equine | |
Rabbit | |
Polyclonal | |
Texas Red |