Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347143

Description

Horse IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Equine
• Application Notes:
  Anti-Horse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Horse IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG and Horse Serum.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat Anti-Horse IgG alkaline phosphatase Conjugated Antibody, goat Anti-Horse IgG Antibody alk phos Conjugation

ELISA Results of Goat Anti-Horse IgG Antibody Alkaline Phosphatase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG (p/n orb2652757). The working dilution is 1:6000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP ELISA Alk Phos Substrate, NPP Buffer, Horse IgG Alk Phos conjugate, and TMB substrate (p/n orb348651).