HIV-1 p24 Recombinant Protein

• Catalog Number: orb1671194
• Category: Proteins
• Description: HIV-1 p24 Recombinant Protein
• Tested applications: ELISA, SDS-PAGE, WB
• Tag: His tag at C-terminus
• Form/Appearance: Liquid
• Purity: > 95% as determined by Bis-Tris PAGE
• MW: 24 kD
• Target: gag
• UniProt ID: P04591
• Protein Sequence: Full length of HIV-1 Gag p24 protein
• Source: E. coli
• NCBI: AAB50258
• Storage: Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Buffer/Preservatives: PBS (pH 7.4).
• Alternative names: Gag polyprotein, Pr55Gag, MA, Caspid p24
• Note: For research use only
• Application notes: This recombinant protein can be used for WB and ELISA. For research use only.
• Expiration Date: 6 months from date of receipt.

ELISA Validation of HIV-1 p24 Recombinant Protein. Coating Antigen: HIV-1 p24 protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: HIV-1 p24 antibodies, orb1239709, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of HIV-1 p24 Recombinant Protein. Loading: 0.5 μg (lane 1) and 1 μg (lane 2) of HIV-1 p24 recombinant protein, orb1671194. Observed: 24kD

WB Validation of HIV-1 p24 Protein with HIV-1 p24 Antibodies. Loading: 5 ng, 10 ng and 25 ng of HIV-1 p24 protein. Detection Antibodies: HIV-1 p24 antibody, orb1239709, 0.5 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr.