HIV-1 p24 Recombinant Protein

SKU: orb1671194

Description

HIV-1 p24 Recombinant Protein

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, SDS-PAGE, WB
• Application Notes:
  This recombinant protein can be used for WB and ELISA. For research use only.

Key Properties

• Source: E. coli
• Target: gag
• Tag: His tag at C-terminus
• MW: 24 kD
• Purity: > 95% as determined by Bis-Tris PAGE
• Protein Sequence: Full length of HIV-1 Gag p24 protein

Storage & Handling

• Storage: Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS (pH 7.4).
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Gag polyprotein, Pr55Gag, MA, Caspid p24

ELISA Validation of HIV-1 p24 Recombinant Protein. Coating Antigen: HIV-1 p24 protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: HIV-1 p24 antibodies, orb1239709, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of HIV-1 p24 Recombinant Protein. Loading: 0.5 μg (lane 1) and 1 μg (lane 2) of HIV-1 p24 recombinant protein, orb1671194. Observed: 24kD

WB Validation of HIV-1 p24 Protein with HIV-1 p24 Antibodies. Loading: 5 ng, 10 ng and 25 ng of HIV-1 p24 protein. Detection Antibodies: HIV-1 p24 antibody, orb1239709, 0.5 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr.

Quick Database Links

Gene Symbol

gag

UniProt

P04591

RefSeq:AAB50258