Histone H3 (Butyryl-Lys14) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499293
• Category: Antibodies
• Description: Histone H3 (Butyryl-Lys14) Recombinant Rabbit Monoclonal Antibody
• Target: H3C1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H3 (Butyryl-Lys14)
• UniProt ID: P68431
• MW: 15 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Clone Number: R3D9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3K122me2; Butyryl-Histone H3(Lys14); Butyryl-Hist
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): HeLa, (+): HeLa + sodium butyrate, (30 mM, 4 hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): MCF-7 + serum starvation (14 hr), (+): MCF-7 + sodium butyrate (50 mM, 24 hr)+, trichostain A (500 ng/ml, 4 hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Neuro-2a, 2: BRL, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.