Hirudin

SKU: orb1909566

Description

Hirudin is a thrombin inhibitor that improves Gsdmd-mediated pyroptosis by inhibiting irf2. It can be used in research on diabetic nephropathy.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 113274-56-9
• MW: 1468.52
• Purity: 99.75%
• Formula: C₆₆H₉₃N₁₃O₂₅
• SMILES: C([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC1=CC=CC=C1)NC([C@@H](NC(CN)=O)CC(O)=O)=O)=O)CCC(O)=O)=O)CCC(O)=O)=O)[C@H](CC)C)(=O)N2[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CC3=CC=C(O)C=C3)C(N[C@H](C(N[C@@H](CCC(N)=O)C(O)=O)=O)CC(C)C)=O)=O)CCC(O)=O)=O)CCC(O)=O)=O)CCC2
• Target: Thrombin

Bioactivity

• In Vivo:
  Hirudin improve the survival of andomized skin flaps in rats while significantly reducin the inflammatory response.Hirudin contributes to wound healing in SD rats after laser surgery. In a mouse model of unilateral ureteral obstruction (UUO), Hirudin (10 and 15 mg/kg; gavage once daily for 21 days) reduced tubular injury and inflammation by ameliorating interstitial fibrosis.
• In Vitro:
  Hirudin prevents fibrin formation by inhibiting thrombin activity and impairing its ability to cleave fibrinogen, and inhibit the process of cross-linking and polymerization of fibrin monomers in the internal and external coagulation pathways. High doses of Hirudin reduc the proliferation of human microvascular endothelial cells by inhibitin the VEGF-Notch pathway. Hirudin (3-10 mg/mL) reverses TGF-β1-induced aberrant proliferation and fibrosis of HK-2 cells Hirudin is also effective in reducin the proliferation of human microvascular endothelial cells. Hirudin inhibits angiotensin II-induced cardiac fibroblast activity in a dose-dependent manner, attenuates oxidative stress, modulates factors associated with fibrosis, and inhibit the ERK1/2 signaling pathway.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hirudin (54-65; non-sulfated)

Compound Identifiers

PubChem CID

16138839