HDAC1 Rabbit Polyclonal Antibody

• Catalog Number: orb704388
• Category: Antibodies
• Description: HDAC1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human HDAC1 (393-482/482aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:100, IF=1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: HDAC1
• UniProt ID: Q13547
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HD 1; HD1; HDAC 1; hdac1: histone deacetylase 1; H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) HDAC1 polyclonal Antibody, Unconjugated (orb704388) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

L929 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) HDAC1 polyclonal Antibody, Unconjugated (orb704388) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (orb704388) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (orb704388) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (orb704388) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: HDAC1 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-HDAC1 (orb704388) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 62 kD, Observed band size: 65 kD.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Molt-4 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-HDAC1 (orb704388) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.