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HDAC1 (phospho Ser421) rabbit pAb

Catalog Number: orb768604

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb768604
CategoryAntibodies
DescriptionRabbit polyclonal antibody to HDAC1 (phospho-Ser421)
ClonalityPolyclonal
Tested applicationsELISA, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human HDAC1 around the phosphorylation site of Ser421. AA range:387-436
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
ConjugationUnconjugated
MW62
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-HDAC1 antibody, anti-RPD3L1 antibody, anti-Hi
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NoteFor research use only
Expiration Date12 months from date of receipt.
HDAC1 (phospho Ser421) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC1 (Phospho-Ser421) Antibody.

HDAC1 (phospho Ser421) rabbit pAb

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.

HDAC1 (phospho Ser421) rabbit pAb

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).