HADA Hydrochloride

SKU: orb1697022

Description

HADA Hydrochloride is a blue-fluorescent D-amino acid probe that covalently incorporates into bacterial peptidoglycan at sites of active synthesis. It enables specific, minimally perturbative visualization of bacterial growth in both in vitro and in vivo research applications, including studies of cell wall dynamics and antibiotic action.

Research Area

Cell Biology, Infectious Disease & Virology

Key Properties

• CAS Number: 2253733-10-5
• MW: 328.71
• Purity: 98.51% (May vary between batches)
• Formula: C₁₃H₁₃ClN₂O₆
• SMILES: Cl.N[C@H](CNC(=O)c1cc2ccc(O)cc2oc1=O)C(O)=O
• Target: Antibacterial,Autophagy
• Solubility: DMSO:112.5 mg/mL (342.25 mM)

Bioactivity

• In Vitro:
  FDAA labeling can be completed in as little as 30 seconds for rapidly growing species like Escherichia coli. Despite HADA Hydrochloride being dimmer and less photostable compared to FDL or TDL, it consistently and effectively labels the PG of a wide range of bacterial species, usually without requiring significant optimization.
• Cell Research:
  HADA labeled PG site experiment of E. coli cells I. Solution preparation Mother solution preparation: Prepare 50 mM (100×) stock solution with DMSO; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) II. Operation steps 1. Inoculate cells from -80°C bacterial cryopreservation solution into sterile liquid culture medium and culture cells using optimal species-specific conditions until exponential growth is achieved. 2. Add 10 μL of 50 mM HADA DMSO stock solution to the culture tube. 3. Dilute the culture to OD600 = 0.05 in fresh LB; 4. Add 990 μL of the culture to the culture tube containing FDA to obtain a final LB solution of [HADA] = 500 μM and [DMSO] = 1%, and incubate the culture at 37°C with shaking; 5. When the culture reaches OD600 = 0.4 – 0.6, pellet the cells (1 min, maximum speed in a microcentrifuge, room temperature); 6. Resuspend the cells in 1 mL 70% (vol/vol) ice-cold EtOH and fix the cells on ice for 10 – 15 min.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HADA HCl, HADA Hydrochloride, HCC-Amino-D-alanine hydrochloride, HCC-Amino-D-alanine Hydrochloride

Compound Identifiers

PubChem CID

137919867