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Catalog Number | orb1671571 |
---|---|
Category | Antibodies |
Description | H3K27me3 Antibody [BT164], Rabbit IgG |
Clonality | Recombinant |
Clone Number | BT164 |
Tested applications | ELISA, ICC, IF, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | trimethyl-Histone H3 (Lys27), histone H3, H3K27me3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of fixed HeLa cells with anti-H3K27me3 antibody BT164. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of BT164 (orb1671571) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671571- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western Blot using anti-H3K27me3 antibody BT164 (Nuclear lysate of HeLa(A) (0.0003 ug/ml)- Jurkat(B) (0.0003 ug/ml) and K562(C) (0.01 ug/ml) (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of BT164 (orb1671571) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the anti-H3K27me3 antibody BT164 HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of BT164 (orb1671571 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.