H2AFX Antibody (C-term)

SKU: orb1927418

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC: 1:50, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 938CT5.1.1
• Target: This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
• Molecular Weight: 15145 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX

Immunohistochemical analysis of paraffin-embedded Human Thymus section using Pink1. Diluted at 1: 50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. thymus section using H2AFX Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line (from left to right). using H2AFX Antibody (C-term). Diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Quick Database Links

Gene Symbol

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.

UniProt

P16104

RefSeq (Protein):NP_002096.1