Guinea Pig IgG F(c) Biotin Antibody

SKU: orb346149

Description

Guinea Pig IgG F(c) Biotin Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT, SDS-PAGE
• Application Notes:
  Guinea Pig IgG F(c) fragment Biotin conjugation has been tested in SDS-Page and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging utilizing various commercial platforms.

Key Properties

• Source: Guinea Pig
• Biological Origin: Guinea Pig
• Isotype: IgG F(c)
• Purity: This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(c) and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(ab’)2 or anti-Papain.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Guinea Pig IgG F(c) fragment Biotin conjugation, Guinea Pig IgG Fc fragment BAC conjugate

SDS PAGE Results of Guinea Pig IgG F(c) Biotin Conjugated. Guinea Pig IgG F(c) Biotin Conjugated (1) 100 ng, (2) 33.33 ng, (3), 11.11 ng, (4) 3.70, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) at RT for 90 mins. Exposure: 5 secs.