Guanidine

SKU: orb2646003

Description

Guanidine is a potent chaotrope widely used to denature proteins in vitro. It is a key reagent in biochemistry and molecular biology research for studying protein unfolding, solubilizing inclusion bodies, and investigating protein folding pathways.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 113-00-8
• MW: 59.07
• Purity: 97.00% (May vary between batches)
• Formula: CH₅N₃
• SMILES: N=C(N)N
• Target: Others

Bioactivity

• In Vivo:
  Two injections of Guanidine at 145 mg/kg per injection is significantly reducing in the death rate of infant mice infected with ten LD50 of coxsackievirus A16 is observed when they are treated 58 h after infection.
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• In Vitro:
  Guanidine at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine (> 1 M) causes co-operative unfolding of the molten globule state. Guanidine at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Guanidine

Compound Identifiers

PubChem CID

3520