GSTM3 Antibody / Glutathione S-Transferase mu 3

• Catalog Number: orb2642133
• Category: Antibodies
• Description: Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. There are eight families of GST proteins, each of which are composed of proteins that have a variety of functions throughout the cell. The GSTM proteins (GSTM1-GSTM5 in human and GSTM1-GSTM7 in mouse) are members of the mu class of enzymes that conjugate with glutathione and function in the detoxification of carcinogens, environmental toxins and products of oxidative stress. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. GSTM3 protein is selectively expressed in the testis and brain. GSTM3 may also be tumor suppressive in RCC, as low expression has been demonstrated to correlate with RCC risk and poor prognosis in post-op patients.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-GSTMu3-1
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for this GSTM3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This GSTM3 antibody is available for research use only.
• UniProt ID: P21266
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the GSTM3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using GSTM3 antibody (clone CPTC-GSTMu3-1). These results demonstrate the foremost specificity of the CPTC-GSTMu3-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.