GSK2200150A

SKU: orb1302781

Description

GSK2200150A

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 1443138-53-1
• MW: 357.47
• Purity: 99.24%
• Formula: C₂₀H₂₃NO₃S
• SMILES: C(N1CCC2(CC1)OCCc1sccc21)c1ccc2OCCOc2c1
• Target: Antibacterial
• Solubility: DMSO:45 mg/mL (125.88 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.59 mM)

Bioactivity

• Target IC50:
  M. tuberculosis (strain H37Rv):0.38 μM (MIC)
• In Vitro:
  GSK2200150A, a novel agent with a spirocycle core, exhibits strong antimycobacterial properties against the virulent Mycobacterium tuberculosis strain (H37Rv), demonstrating a minimal inhibitory concentration (MIC) of 0.38 μM .
• Cell Research:
  GSK2200150A is tested for activity at either single concentration (100 μM) or serially diluted in 10 μL of purified H2O in triplicate in 96 well microtiter plates. M. tuberculosis H37Rv is grown in complete Middlebrook 7H9 media containing albumin, dextrose and catalase (ADC), 20% Tween 80 and 50% glycerol. A bacterial suspension (90 μL) at OD600 nm of 0.001 is added to the wells and incubated for 7 days. Resazurin (10 μL; 0.05%(w/v)) is then added, incubated for 24 h at 37°C, and fluorescence measured at 590 nm using a FLUOstar Omega microplate reader. After subtraction of background fluorescence from all wells, the percentage mycobacterial survival is determined by comparing the fluorescence of wells containing compounds compared to control wells not treated with compound.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, GSK2200150A, GSK-2200150A, Inhibitor, Bacterial

Compound Identifiers

PubChem CID

70689288