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Catalog Number | orb1257525 |
---|---|
Category | Antibodies |
Description | GP9 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 17-147 of human GP9 (NP_000165.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 19kDa |
Target | GP9 |
UniProt ID | P14770 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Platelet glycoprotein IX, GP-IX, GPIX, Glycoprotei Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using GP9 antibody (orb1257525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using GP9 antibody (orb1257525). Blue: DAPI for nuclear staining.