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Goat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

Catalog Number: orb779698

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779698
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha(TNFa).
Tested applicationsELISA
ReactivityGoat
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
UniProt IDP13296
Sensitivity5.9 pg/mL
Alternative namesDIF ELISA Kit, TNF-A ELISA Kit, TNFSF2 ELISA Kit,
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Goat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

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