UCP1 Antibody

SKU: orb19218

Description

Goat polyclonal antibody to UCP1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.5-1 μg/ml
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 35kDa band observed in Human Adipose lysates (calculated MW of 33.0kDa according to NP_068605.1). Recommended concentration: 0.1-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: UCP1
• Protein Sequence: EQLKRELSKSRQ
• Molecular Weight: 33
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti UCP1 antibody, anti uncoupling protein 1 (mitochondrial, proton carrier) antibody, anti HGNC:12517 antibody, anti SLC25A7 antibody, anti UCP antibody, anti mitochondrial brown fat uncoupling protein antibody, anti uncoupling protein 1 antibody

orb19218 (1ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

UCP1

NCBI Gene ID

7350