UCP1 Antibody

• Catalog Number: orb19218
• Category: Antibodies
• Description: Goat polyclonal antibody to UCP1
• Target: UCP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EQLKRELSKSRQ
• RRID: AB_10749109
• MW: 33
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.5-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 35kDa band observed in Human Adipose lysates (calculated MW of 33.0kDa according to NP_068605.1). Recommended concentration: 0.1-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UCP1 antibody, anti uncoupling protein 1 (mit
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 7350
• Expiration Date: 12 months from date of receipt.

orb19218 (1ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.