Goat anti-TBK1 (aa514-527) Antibody

• Catalog Number: orb137099
• Category: Antibodies
• Description: Goat polyclonal to TBK1
• Target: TBK1 (aa514-527)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1-3 μg/ml
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TIETSLQDIDSRLS
• MW: 83.6
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FLJ11330 antibody, anti NAK antibody, anti NF
• Note: For research use only
• Entrez: 29110

Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using TBK1 antibody