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Catalog Number | orb20322 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TLN1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:8000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 270 |
Target | Talin 1 / TLN1 |
Entrez | 7094 |
Protein Sequence | QEALHTQMLTAVQE |
RRID | AB_10752698 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TLN1 antibody, anti talin 1 antibody, anti IL Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: A > 250kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 270kDa according to NP_006280.3). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 110kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb20322 (1ug/ml) staining of Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.