Talin 1/TLN1 Antibody

SKU: orb20322

Description

Goat polyclonal antibody to TLN1

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: A > 250kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 270kDa according to NP_006280.3). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 110kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Talin 1 / TLN1
• Protein Sequence: QEALHTQMLTAVQE
• Molecular Weight: 270
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TLN1 antibody, anti talin 1 antibody, anti ILWEQ antibody, anti KIAA1027 antibody, anti TLN antibody

orb20322 (1ug/ml) staining of Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Talin 1 / TLN1

NCBI Gene ID

7094