Talin 1/TLN1 Antibody

• Catalog Number: orb20322
• Category: Antibodies
• Description: Goat polyclonal antibody to TLN1
• Target: Talin 1 / TLN1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QEALHTQMLTAVQE
• RRID: AB_10752698
• MW: 270
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: A > 250kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 270kDa according to NP_006280.3). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 110kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TLN1 antibody, anti talin 1 antibody, anti IL
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 7094

orb20322 (1ug/ml) staining of Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.