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Goat anti-PTPN6 / SHP1 (internal) Antibody

SKU: orb19494

Description

Goat polyclonal antibody to PTPN6

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:128000, WB: 0.2-0.5 μg/ml
ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in lysates of cell lines Jurkat and U937 (calculated MW of 67.7kDa according to NP_536858.1 and 67.6kDa according to NP_002822.2). Recommended concentration: 0.2-0.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetPTPN6 / SHP1 (internal)
Protein SequenceHTKNKREEKVKKQ
Molecular Weight67.7; 67.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PTPN6 antibody, anti SHP1 antibody, anti protein tyrosine phosphatase, non-receptor type 6 antibody, anti HCP antibody, anti HCPH antibody, anti HPTP1C antibody, anti PTP-1C antibody, anti SH-PTP1 antibody, anti SHP-1 antibody, anti SHP-1L antibody, anti 70 kda SHP-1L protein antibody, anti hematopoietic cell phosphatase antibody, anti hematopoietic cell protein-tyrosine phosphatase antibody, anti protein-tyrosine phosphatase 1C antibody
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Goat anti-PTPN6 / SHP1 (internal) Antibody

orb19494 (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Goat anti-PTPN6 / SHP1 (internal) Antibody (orb19494)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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