Goat anti-PAM / PAL Antibody

• Catalog Number: orb19751
• Category: Antibodies
• Description: Goat polyclonal to PAM
• Target: PAM / PAL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DQEKEDDGSESEE
• RRID: AB_10749246
• MW: 108; 101; 96.3; 98.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 0.3-1 μg/ml
• Application notes: This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 100kDa band observed in Human Kidney lysates (calculated MW of 101kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PAM antibody, anti PAL antibody, anti peptidy
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 5066

orb19751 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.