P2RY1 (aa247-257) Antibody

SKU: orb19716

Description

Goat polyclonal antibody to P2RY1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:128000, WB: 0.1 μg/ml
• Predicted Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: P2RY1 (aa247-257)
• Protein Sequence: KDLDNSPLRRK
• Molecular Weight: 42.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti P2RY1 antibody, anti purinergic receptor P2Y, G-protein coupled, 1 antibody, anti P2Y1 antibody, anti ATP recepto antibody, anti P2 purinoceptor subtype Y1 antibody, anti P2Y purinoceptor 1 antibody, anti platelet ADP receptor antibody, anti purinergic receptor P2Y1 antibody

Quick Database Links

Gene Symbol

P2RY1 (aa247-257)

NCBI Gene ID

5028