Goat anti-NEFL (aa330-343) Antibody

• Catalog Number: orb1796
• Category: Antibodies
• Description: Goat polyclonal antibody to NEFL
• Target: NEFL (aa330-343)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EKQLQELEDKQNAD
• MW: 61.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 0.3-1 μg/ml
• Application notes: WB: Approx 68-70kDa band observed in lysates of Neuroblastoma cell line KELLY (calculated MW of 61.5kDa according to NP_006149.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NEFL, neurofilament, light polypeptide, CMT1F, CMT
• Note: For research use only
• Entrez: 4747

Western blot analysis of KELLY lysate using NEFL antibody