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NAC1/BTBD14B Antibody

SKU: orb19457

Description

Goat polyclonal antibody to BTBD14B

Research Area

Epigenetics, Stem Cells

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:2000, WB: 0.5-1.5 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 57.3kDa according to NP_443108.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNAC1 / BTBD14B
Protein SequenceKTEQQESDSVQC
Molecular Weight57.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti BTBD14B antibody, anti NAC1 antibody, anti BTB (POZ) domain containing 14B antibody, anti FLJ37383 antibody, anti transcriptional repressor NAC1 antibody

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NAC1/BTBD14B Antibody

orb19457 (0.5ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NAC1/BTBD14B Antibody (orb19457)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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