MELK Antibody

• Catalog Number: orb18300
• Category: Antibodies
• Description: Goat polyclonal antibody to MELK
• Target: MELK
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KRLVEDILSSCKV
• RRID: AB_10755412
• MW: 74.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MELK antibody, anti maternal embryonic leucin
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 9833

Western blot analysis of human testis lysate using MELK antibody