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Catalog Number | orb20401 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to LPP |
Target | LPP |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Bovine, Canine, Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | RNDSDPTYGQQGHP |
RRID | AB_10750598 |
MW | 65.7 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 85kDa band observed in Human Placenta lysates and in lysates of cell line HepG2 (calculated MW of 65.7kDa according to NP_005569.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti LPP antibody, anti LIM domain containing pref Read more... |
Note | For research use only |
Entrez | 4026 |
orb20401 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.