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Goat anti-Human IgG Fc, HRP conjugated
SKU: orb700331
Description
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | The optimal working dilution should be determined by the investigator |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Purified human IgG |
| Purity | Immunogen affinity purified goat IgG. |
| Purification | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
|---|---|
| Form/Appearance | Lyophilized |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-Human IgG Fc, HRP conjugated (orb700331)
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