Goat anti-HCAP-H Antibody

• Catalog Number: orb19516
• Category: Antibodies
• Description: Goat polyclonal to HCAP-H
• Target: HCAP-H
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TEDLSDVLVRQGD
• RRID: AB_10748408
• MW: 82.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell. 2001 Nov;12(11):3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HCAP-H antibody, anti NCAPH antibody, anti no
• Note: For research use only
• Entrez: 23397

orb19516 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.