ERK2/MAPK1 Antibody

SKU: orb18945

Description

Goat polyclonal antibody to MAPK1

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ERK2 / MAPK1
• Protein Sequence: CAAGPEMVRGQVF
• Molecular Weight: 41.4; 41.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MAPK1 antibody, anti ERK2 antibody, anti ERK antibody, anti p38 antibody, anti p40 antibody, anti p41 antibody, anti ERT1 antibody, anti MAPK2 antibody, anti PRKM1 antibody, anti PRKM2 antibody, anti P42MAPK antibody, anti p41mapk antibody, anti mitogen-activated protein kinase 1 antibody, anti protein tyrosine kinase ERK2 antibody, anti mitogen-activated protein kinase 2 antibody, anti extracellular signal-regulated kinase 2 antibody

orb18945 (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ERK2 / MAPK1

NCBI Gene ID

5594