Choline acetyltransferase Antibody

SKU: orb18940

Description

Goat polyclonal antibody to Chat

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 0.03-0.3 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Choline acetyltransferase
Protein Sequence	CKEKATRPSQGHQP
Molecular Weight	70.4; 82.6; 74.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-Acetyl CoA choline O acetyltransferase antibody, anti- Acetyl CoA:choline O acetyltransferase antibody, anti- ChAT antibody, anti- CHOACTase antibody, anti- Choline acetylase antibody, anti- choline acetyltransferase antibody, anti- Choline O acetyltransferase antibody, anti- Choline O-acetyltransferase antibody, anti- CLAT_HUMAN antibody, anti- CMS1A antibody, anti- CMS1A2 antibody, anti- EC 2.3.1.6 antibody, anti- OTTHUMP00000019583 antibody, anti- OTTHUMP00000019584 antibody

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orb18940 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Choline acetyltransferase

NCBI Gene ID

1103

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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