BRSK2/STK29 Antibody

SKU: orb19240

Description

Goat polyclonal antibody to BRSK2

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:8000, WB: 0.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave bands at approx 50-55kDa and 15kDa in human brain, mouse brain and rat brain lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BRSK2 / STK29
• Protein Sequence: CPEVIRGEKYDGRKAD
• Molecular Weight: 74.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BRSK2 antibody, anti SAD1 antibody, anti STK29 antibody, anti PEN11B antibody, anti C11orf7 antibody, anti BR serine/threonine kinase 2 antibody, anti serine/threonine kinase 29 antibody, anti homolog of SAD-1 (C. elegans) antibody, anti chromosome 11 open reading frame 7 antibody, anti chromsosome 11 open reading frame 7 antibody, anti FLJ41362 antibody, anti protein kinase SAD1B antibody, anti serine/threonine kinase SAD-A antibody

Quick Database Links

Gene Symbol

BRSK2 / STK29

NCBI Gene ID

9024