GNE-371

SKU: orb1707258

Description

GNE-371 is a selective chemical probe targeting the second bromodomain of human TAF1 and TAF1L, subunits of transcription factor TFIID. It is used in epigenetic research to study gene regulation, with applications in cancer biology and neurodevelopmental studies, primarily in vitro and in cellular models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 1926986-36-8
• MW: 431.49
• Purity: 98.21%
• Formula: C₂₄H₂₅N₅O₃
• SMILES: Cn1cnc2c(cc(cc12)C(=O)N1CCOCC1)-c1cn(CCC=C)c(=O)c2[nH]ccc12
• Target: DNA/RNA Synthesis
• Solubility: DMSO:30 mg/mL (69.53 mM)

Bioactivity

• Target IC50:
  TAF12:10 nM
• In Vitro:
  Instructions 1. Solvent selection: GNE-371 is soluble in organic solvents, such as anhydrous dimethyl sulfoxide (DMSO) or acetonitrile. A solution with a concentration of 1–10 mM is usually used, and the specific concentration can be adjusted according to experimental requirements. 2. Labeling reaction: Mix the GNE-371 solution with the molecule to be labeled (such as an antibody or protein). The reaction is usually carried out in a buffer of about pH 8.0, the reaction temperature is generally room temperature, and the time is usually 1–2 hours. 3. Reaction termination and purification: After the reaction is completed, dialysis or gel filtration columns can be used to remove unbound GNE-371 to ensure that the labeled molecules are pure and avoid background signals. 4. Detection and analysis: Use appropriate detection methods, such as fluorescence microscopy, flow cytometry, or fluorescence spectrophotometer to analyze the fluorescence signal of the labeled sample. The excitation wavelength of GNE-371 is 649 nm and the emission wavelength is 667 nm.

Storage & Handling

• Storage: store under nitrogen,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TAF12, RNA Synthesis, RNASynthesis, GNE371, GNE-371, GNE 371, DNA/RNA Synthesis, DNA Synthesis, DNASynthesis

Compound Identifiers

PubChem CID

121335283