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GOLGA2 Antibody
SKU: orb1257543
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3). |
| Target | GOLGA2 |
| Molecular Weight | Observed: 145kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GM13golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgi matrix protein GM13SY11 protein, golgi autoantigen, golgin subfamily a, 2, golgin-95
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Confocal immunofluorescence analysis of Hela cells using GM130 antibody (orb1257543) at dilution of 1:400. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GM130 antibody (orb1257543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using GM130 antibody (orb1257543) at dilution of 1:400 (60x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug GM130 antibody (orb1257543). Western blot was performed from the immunoprecipitate using GM130 antibody (orb1257543) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
GOLGA2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
GOLGA2 Antibody (orb1257543)
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