Genistein

SKU: orb1307572

Description

Genistein

Research Area

Cardiovascular Research

Key Properties

• CAS Number: 446-72-0
• MW: 270.24
• Purity: >98%
• Formula: C₁₅H₁₀O₅
• SMILES: O=C1C(=COC=2C=C(O)C=C(O)C12)C=3C=CC(O)=CC3
• Target: GPCR
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 4 mg/ml).

Bioactivity

• Target IC50:
  EGFR:0.6 μM (EC50)|ME-180 cells:60 µM|MRGPRX2:38.3 μM (EC50)|SW620 cells:152.60 ± 19.13 µM|MCF-7 cells:10 μM|MDA-MB-231 cells:10 μM|HeLa cells:35 µM|HaCaT cells:225.01 ± 7.06 µM|Topoisomerase II:25-80 µm (EC50)|HBL-100 cells:10 μM|SW480 cells:128.80 ± 15.87 μM|CaSki cells:24 µM
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• In Vivo:
  METHODS: To assay antitumor activity in vivo, Genistein (2-20 mg/kg in 25 mmol/L Na2CO3) was administered by gavage to B6C3F1 mice bearing B16F10 tumors once daily for 28 days. RESULTS: Consistent with the chemopreventive effects of Genistein, exposure to this compound significantly increased host resistance to B16F10 tumors, which was reflected in a reduction in the number of lung tumor nodules after tumor cell injections at moderate to high dose levels.
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• In Vitro:
  METHODS: Breast cancer cells MCF-7 were treated with Genistein (10-200 µM) for 24-48 h. Cell viability was measured by MTT assay. RESULTS: Genistein was cytotoxic to MCF-7 cells treated with Genistein at a concentration of more than 80 µM for 24 hours. Treatment with Genistein above 50 µM for 48 h induced cytotoxicity in MCF-7 cells. METHODS: MCF-7 cells or differentiating 3T3-L1 cells were treated with Genistein (50-200 µM) for 24-72 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: In MCF-7 cells, all concentrations of Genistein down-regulated ERα expression; furthermore, the effect of Genistein was greater after 48 and 72 h. Compared with the negative control (3T3-L1 preadipocytes), the expression of ERα was upregulated 1.98-fold in 3T3-L1 cells after 48 h of Genistein treatment to initiate differentiation.
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• Cell Research:
  Genistein is dissolved in DMSO and stored, and then diluted with appropriate medium before use. The IC50 values for Genistein are determined by the MTT assay. Briefly, the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO, and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4’,5,7-Trihydroxyisoflavone

Compound Identifiers

PubChem CID

5280961