General S-Adenosyl Methionine (SAM) ELISA Kit

• Catalog Number: orb1946698
• Category: Assays and Kits
• Description: General S-Adenosyl Methionine (SAM) ELISA Kit
• Range: 15.63-1000 ng/mL
• Assay Time: 1.5H
• Target: SAM
• Sensitivity: 4.84 ng/mL
• Alternative names: SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S-Adenosyl Methionine(SAM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S-Adenosyl Methionine(SAM). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Adenosyl Methionine(SAM) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.