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General Gamma-Aminobutyric Acid (gABA) ELISA Kit
Catalog Number: orb775377
| Catalog Number | orb775377 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gamma-Aminobutyric Acid(gABA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-Aminobutyric Acid(gABA). |
| Reactivity | All |
| Concentration | 2000 pg/mL |
| Tested applications | ELISA |
| Application notes | standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of gABA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 31.25-2000 pg/mL |
| Sensitivity | 9.4 pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | 4-Aminobutyric Acid ELISA Kit |
| Research Area | Cardiovascular Research, Musculoskeletal & Connect Read more... |
| Note | For research use only |
| Expiration Date | Please enquire. |
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